Microbiology

12 Doctors

Day Timings unit HOD/Unit Chief&Faculty
Monday to Saturday 9.00 am to 4.00 pm I Dr. Sumana M N

Department of Microbiology is mainly involved in Teaching, Research and Diagnostic services. Teaching is mainly focused on applied aspects of Clinical Microbiology.

Bacteriology

  • Abscess culture and sensitivity
  • Anaerobic culture and sensitivity
  • Ascitic fluid culture and sensitivity
  • BAL culture and sensitivity
  • Bile culture and sensitivity
  • Blood bag culture and sensitivity
  • Blood culture and sensitivity
  • Bone marrow culture and sensitivity
  • Catheter tip culture and sensitivity
  • CSF culture and sensitivity
  • CSSD Aerobic and anaerobic culture
  • CSSD Biological Indicator culture
  • Cyst fluid culture and sensitivity
  • Drain fluid culture and sensitivity
  • Ear swab culture and sensitivity
  • Eye swab culture and sensitivity
  • Gastric aspirate culture and sensitivity
  • Nasal swab culture and sensitivity
  • OT swab culture and sensitivity
  • Pericardial culture and sensitivity
  • Pus culture and sensitivity
  • Semen culture and sensitivity
  • Sputum culture and sensitivity
  • Stool culture and sensitivity
  • Synovial fluid culture and sensitivity
  • Throat swab culture and sensitivity
  • Tissue culture and sensitivity
  • Urine culture
  • Vaginal swab culture and sensitivity
  • Water analysis
  • Environmental Surveillance culture

Microscopy

  • AFB /ZN stain
  • Albert stain
  • Gram stain
  • India ink preparation
  • Modified ZN stain
  • PCP – Toluidine Blue stain
  • Slit skin smear
  • Stool hanging drop

Serology – General

  • Brucella Standard Agglutination Test (SAT)
  • Cold Agglutination Test
  • Paul Bunnel Test
  • VDRL / RPR
  • Weil Felix test
  • Widal test
  • Procalcitonin test

Serology – Immunology

  • ANA – Immunofluroscence
  • ANA profile
  • ANCA
  • Anti CCP
  • ASLO
  • CRP
  • DS DNA
  • PSA
  • RA
  • Total IgE
  • SSA Autoantibody ELISA
  • SSB Autoantibody ELISA
  • Anti Cardiolipin Screen
  • Anti beta 2 Glycoprotein 1 screen
  • Aspergillus Galactomannan antige
  • Scrub typhus ELISA

Serology – Virology

  • Anti HAV
  • Anti HBC IgM
  • Anti HBE IgM
  • Anti HBSAg
  • Anti HCV
  • Anti HEV
  • Anti HIV
  • Chikungunya IgM
  • Dengue Serology
  • HBeAg
  • HBsAg

Parasitology

  • MP – PBS
  • Malaria card test
  • Stool Microscopy
  • Wet mount preparation

Mycology

  • KOH mount
  • Fungal culture

Molecular diagnostics

  • COVID 19 RTPCR
  • H1N1 RT PCR
  • Influenza RTPCR
  • CBNAAT for MDR TB and XDR TB

  • Diagnosis of Bacterial Infectious Diseases including TB
  • Viral infectious diseases like HIV
  • Hepatitis
  • Dengue Influenza (A, B and H1N1)
  • COVID 19
  • Fungal infectious diseases such as yeasts
  • Molds
  • Parasitic infectious diseases
  • Autoimmune diseases

CULTURE & SENSITIVITY

  • Aerobic bacteria and Yeast
  • Anerobic bacteria
  • Mycobacteria
  • Fungal Culture

GENERAL SEROLOGICAL TESTS

  • ASO
  • Cold agglutination test
  • VDRL-RPR
  • WIDAL
  • Weil Felix
  • Paul Bunnel
  • Brucella Agglutination test
  • TPHA

IMMUNOCHROMATOGRAPHIC TESTS

  • Dengue- NS-1/IgG/IgM
  • Leptospira- IgG/IgM
  • HBsAg
  • ANTI HIV
  • ANTI HCV
  • ANTI HAV
  • ANTI HEV
  • Toxoplasma IgM
  • Clostridium difficle Toxin/Antigen
  • Malaria HRP2/LDH

SPECIAL INVESTIGATIONS

  • Water surveillance
  • Surveillance cultures

DEPARTMENTS

  • ART
  • SRL
  • Viral Load
  • Molecular Diagnostic Lab
  • RNTCP
  • ICTC

ELISA

  • Anti HAV
  • HBsAg
  • ANTI HCV
  • ANTI HEV
  • HbeAg
  • Anti HbcIgM
  • Anti HBs
  • Total IgE
  • DENGUE
  • PSA
  • Leptospira
  • Anti HbeAg
  • Cryptosporidium antigen

MICROSCOPY

  • Gram stain
  • Ziehl Neelsen stain
  • Albert stain
  • Toluidine blue stain
  • Modified acid fast stain
  • Stool Microscopy
  • KOH
  • DGM
  • India ink
  • Calcoflour white
  • Slit skin smear
  • Peripheral blood smear
  • Hanging drop
  • QBC
  • Pneumoslide

CLINICAL IMMUNOLOGY

  • CRP
  • RA
  • ANA
  • dsDNA
  • ANCA (cANCA, pANCA)
  • ANA Profile
  • ANTI CCP

LIST OF INVESTIGATION DONE IN MOLECULAR LAB

Sl. No. Panel Name
1 VIRAL ENCEPHALITIS PANEL
2 MENINGOENCEPHALITIS PANEL
3 STD PANEL
4 HPV HR GENOTYPEING
5 TROPICAL FEVER / PANEL
6 Malarial Parasite (MP) PCR
7 NEONATAL SEPSIS
8 SEPSIS (MULTIPLEX TANDEM PCR)
9 GASTROENTERITIS – VIRAL
10 GASTROENTERITIS – PARASITE
11 PAN FUNGAL PCR
12 FLU Panel (Subtyping)
13 RESPIRATORY INFECTIONS COMBINED PANEL / VIRUSES / BACTERIA
14 Pneumocystis jirovecii PCR
15 MTB MDR Susceptibility PCR
16 MTB XDR Susceptibility (PCR)
17 MTB/MOTT DNA PCR
18 MTB DNA PCR
19 HBV DNA / QUANTITATIVE
20 HIV 1 RNA QUANTITATIVE
21 HCV RNA GENOTYPING / QUALITATIVE
22 CMV QUALITATIVE / QUANTITATIVE
23 HLA B 27
24 EBV PCR
25 Aspergillus sp. PCR
26 Vector borne Screning
27 Adenovirus PCR
28 C.diptheriae toxin A&B PCR
29 TORCH PCR
30 Cryptococcus PCR
31 Chlamydia pnemoniae PCR

NAME OF EQUIPMENT
Walk in Cold Room
Incubator – 1
Laminar Airflow
Bio- safety Cabinet
Centrifuges
Auto Claves
Vitek
Analytical Weighing Balance
Fluorescent Microscopes
Microscope
CLIA Machine
Cyclomixer
Biomeriux – Vitek
BacT – Alert and MB – Bact

  • Study of colonization of methicillin resistant staphylococcus aureus in intensive care unit patient environment and health care workers.
  • Study of direct antibiotic susceptibility from positive monobacterial blood culture
  • Comparison of conventional and molecular methods in the detection of Mycobacterium tuberculosis in clinically suspected cases of tuberculosis
  • Rate of occurrence of enterically transmitted hepatitis viruses among infective hepatitis cases in a tertiary care hospital
  • Study of seropositivity of syphilis among suspected cases attending atertiary care hospital
  • Comparison of different techniques for the laboratory diagnosis of hepatitis B virus infection
  • Comparison between Glutaraldehyde (Bacillocid) and isopropyl alcohol as surface disinfectant in ICUs of a tertiary care hospital- A Study
  • Role of Antimicrobial susceptibility profile in the phenotypic detection of mecc gene in methicillin resistant Staphylococcus aureus
  • Clinico-Microbiological study of Helicobacter Pylori infection in patients with Acid peptic Disease
  • Molecular Detection of Mycoplasma Pneumoniae in Community Acquired Pneumonia.S
  • Study on preoperative screening for multidrug resistant pathogens and its outcome on surgical site infections
  • Study on biofilm formation and its association with multi drug resistance among salmonella isolates
  • KAP of health care workers who sustain needle stick injuries in a tertiary care hospital
  • Environmental surveillance of operation theaters and intensive care units in atertiary care hospital
  • MTB MDR Susceptibility PCR
  • Nasal carriage rate of methicillin resistant staphylococcus aureus in dialysis patient